Posts Tagged ‘circulating tumor dna’

Radiation Therapy

Tuesday, July 28th, 2009

Radiation therapy is an exciting new technique being used to combat cancer. This fascinating technology is always evolving. It works by damaging the DNA of cells. The damage is caused by directing a beam of photons, protons, electrons, neutrons, or ions which can directly or indirectly ionize the atoms which make up the DNA chain. Although this may seem permanent, cells have mechanisms for repairing DNA after this process has happened, which is why Radiation Therapists now focus on breaking the DNA on each separate strand to ensure the DNA is destroyed. This DNA damage has been observed to be inherited throughout the cell division of tumors leading to cancer cell apoptosis or decrease in the rate of cancer cell division.

While there are also many drawbacks of radiation therapy, the largest one is cells of solid tumors often become deficient in oxygen and cause hypoxia. This causes the radiation to be far less effective because tumor cells in a hypoxic environment have a shown a general resistance to radiation therapy. Currently, researchers are working hard to solve this problem.

By providing researchers with the samples they need, we are hopeful we can contribute to new advances in this exciting field. If you have and question please feel free to comment or fill out the form to the left.

Circulating Tumor DNA | Serum and Plasma | Conversant

Thursday, June 11th, 2009

Tags: Announcements, luke doiron, circulating tumor cells, circulating tumor dna

Conversant is excited to now be able to purify free-circulating DNA and RNA from our human plasma and/or serum samples using QIAGEN’s QIAamp Circulating Nucleic Acid Kit.

To date, our clients have used our expertise and samples in one of two ways:

1. They order our patient cancer serum or cancer plasma – the Circulating Tumor DNA kit can be used on our inventory of plasma or serum aliquots, so that is an easy and immediate way that clients can get started. Starting sample size can range from 1.0mL – 5.0mL aliquots.

2. They engage us to collect samples, run the Circulating Tumor DNA kits, and provide them back with the data – for clients trying to simply get the data they need, our lab technicians are proficient and can run the Circulating Tumor DNA kits and simply return the required data.

Like any other new technology, we always get the question “great, so what can I do with these samples and the circulating tumor DNA kit?” My answer is that by the time you get the purified and concentrated circulating DNA and RNA (which is free of proteins, nucleases, and other impurities… by the way), it is ready to use in wide range of downstream applications like:

* PCR and quantitative real-time PCR and RT-PCR
* Biomarker research and validation for blood-based cancer detection
* Viral nucleic acid detection